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Subsequently, splenocytes were washed by PBS and resuspended at 1 × 10 6 /mL in T-cell medium (formula listed below) containing OVA peptide (1 μg/mL, #SP1050a, Abcepta Biotech, USA) and IL-2 (10 ng/mL, #200-02-500, Pepro Tech, USA) to activate CD8 + T cells